WormBase Tree Display for Expr_pattern: Expr12869
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| Expr12869 | Expression_of | Gene | WBGene00004265 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004265 | ||
| Expression_data | Anatomy_term (13) | ||
| GO_term | GO:0005634 | ||
| GO:0030425 | |||
| GO:0030424 | |||
| GO:0043025 | |||
| Subcellular_localization | In the ASH sensory neurons, the reporter protein uniformly stained the cell body, nucleus, dendrite and axon. The staining was too faint to unambiguously determine whether QUI-1::GFP localized also in the cilia. In most of the other neurons, expression of GFP was lower in all cellular compartments and apparently absent from the nucleus. | ||
| Type | Reporter_gene | ||
| Pattern | qui-1::GFP was visible in the processes of the nerve ring and in several neurons of the lateral and ventral ganglia, including the avoidance sensory neurons ASH and ADL. GFP expression was not detected in ASK sensory neurons. Four additional neurons in the retrovesicular ganglion and two neurons in the lumbar ganglion, PVQ and the sensory neuron PHB, also express GFP. This expression pattern partially overlaps but is not completely coincident with that obtained with a much shorter construct in which GFP was fused to the second exon, and the construct was thus missing part of exon 2 and the last 17 introns and exons of the gene (not shown). Expression from this shorter construct included neither ASH nor PHB, indicating that important regulatory elements are present within the coding region of qui-1. | ||
| Reference | WBPaper00006481 | ||
| Transgene | WBTransgene00031982 |
