WormBase Tree Display for Expr_pattern: Expr12249
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Expr12249 | Expression_of | Gene | WBGene00003330 | ||
---|---|---|---|---|---|
Reflects_endogenous_expression_of | WBGene00003330 | ||||
Expression_data | Life_stage | WBls:0000024 | Anatomy_term | WBbt:0004574 | |
WBbt:0004577 | |||||
WBls:0000027 | Anatomy_term | WBbt:0004574 | |||
WBbt:0004577 | |||||
WBls:0000035 | Anatomy_term | WBbt:0004522 | |||
WBbt:0004574 | |||||
WBbt:0004577 | |||||
WBbt:0005753 | |||||
WBbt:0006760 | |||||
WBbt:0006865 | |||||
WBbt:0006903 | |||||
WBbt:0007809 | |||||
Anatomy_term | WBbt:0004522 | Certain | |||
Life_stage | WBls:0000035 | ||||
WBbt:0004574 | Certain | ||||
Life_stage | WBls:0000024 | ||||
WBls:0000027 | |||||
WBls:0000035 | |||||
WBbt:0004577 | Certain | ||||
Life_stage | WBls:0000024 | ||||
WBls:0000027 | |||||
WBls:0000035 | |||||
WBbt:0005753 | Certain | ||||
Life_stage | WBls:0000035 | ||||
WBbt:0006760 | Certain | ||||
Life_stage | WBls:0000035 | ||||
WBbt:0006865 | Certain | ||||
Life_stage | WBls:0000035 | ||||
WBbt:0006903 | Certain | ||||
Life_stage | WBls:0000035 | ||||
WBbt:0007809 | Certain | ||||
Life_stage | WBls:0000035 | ||||
Not_in_Anatomy_term | WBbt:0008125 | Life_stage | WBls:0000035 | ||
Type | Reporter_gene | ||||
Pattern | mir-237, was not expressed in the seam cells until eL3, which mirrored the up-regulation of this gene at this stage by northern blot. mir-237, was detected two stages earlier at L1 in the somatic gonad progenitor cells, Z1 and Z4. This result differs from our northern blot data, which showed that miR-237 RNA was first detected at L2 (Expr3739). We believe that the mir-237::gfp expression at the L1 stage is accurate, and since mir-237 is only expressed in the Z1 and Z4 cells at this stage, miR-237 RNA is below the levels of detection by northern blot analysis. Once the Z1 and Z4 somatic gonad derivatives expand at L2, miR-237 RNA can be seen by northern blot. By the L3 stage, and persisting into the adult, mir-237 was expressed in the developing gonad, including the anchor cell, a subset of unidentified uterine cells, and the distal tip cells. The up-regulation of mir-237 expression in animals carrying the mir-237::gfp construct at the L3 stage in a variety of tissues, such as the gonad and the hypodermis, mirrors the increased miR-237 RNA levels observed at L3 by northern blot analysis. In addition, mir- 237 and mir- 84, were both expressed in the anchor cell and in the VPCs except P6.p at the mL3 stage. It is interesting to note that expression of mir-237, mir-84, and mir-48 was absent in the P6.p cell during the mid- to late L3 stage, and expression of mir-237 and mir-84 but not mir-48 was present in the anchor cell. | ||||
Reference | WBPaper00026854 | ||||
Transgene | WBTransgene00031792 |