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WormBase Tree Display for Expr_pattern: Expr12249

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Name Class

Expr12249Expression_ofGeneWBGene00003330
Reflects_endogenous_expression_ofWBGene00003330
Expression_dataLife_stageWBls:0000024Anatomy_termWBbt:0004574
WBbt:0004577
WBls:0000027Anatomy_termWBbt:0004574
WBbt:0004577
WBls:0000035Anatomy_termWBbt:0004522
WBbt:0004574
WBbt:0004577
WBbt:0005753
WBbt:0006760
WBbt:0006865
WBbt:0006903
WBbt:0007809
Anatomy_termWBbt:0004522Certain
Life_stageWBls:0000035
WBbt:0004574Certain
Life_stageWBls:0000024
WBls:0000027
WBls:0000035
WBbt:0004577Certain
Life_stageWBls:0000024
WBls:0000027
WBls:0000035
WBbt:0005753Certain
Life_stageWBls:0000035
WBbt:0006760Certain
Life_stageWBls:0000035
WBbt:0006865Certain
Life_stageWBls:0000035
WBbt:0006903Certain
Life_stageWBls:0000035
WBbt:0007809Certain
Life_stageWBls:0000035
Not_in_Anatomy_termWBbt:0008125Life_stageWBls:0000035
TypeReporter_gene
Patternmir-237, was not expressed in the seam cells until eL3, which mirrored the up-regulation of this gene at this stage by northern blot. mir-237, was detected two stages earlier at L1 in the somatic gonad progenitor cells, Z1 and Z4. This result differs from our northern blot data, which showed that miR-237 RNA was first detected at L2 (Expr3739). We believe that the mir-237::gfp expression at the L1 stage is accurate, and since mir-237 is only expressed in the Z1 and Z4 cells at this stage, miR-237 RNA is below the levels of detection by northern blot analysis. Once the Z1 and Z4 somatic gonad derivatives expand at L2, miR-237 RNA can be seen by northern blot. By the L3 stage, and persisting into the adult, mir-237 was expressed in the developing gonad, including the anchor cell, a subset of unidentified uterine cells, and the distal tip cells. The up-regulation of mir-237 expression in animals carrying the mir-237::gfp construct at the L3 stage in a variety of tissues, such as the gonad and the hypodermis, mirrors the increased miR-237 RNA levels observed at L3 by northern blot analysis. In addition, mir- 237 and mir- 84, were both expressed in the anchor cell and in the VPCs except P6.p at the mL3 stage. It is interesting to note that expression of mir-237, mir-84, and mir-48 was absent in the P6.p cell during the mid- to late L3 stage, and expression of mir-237 and mir-84 but not mir-48 was present in the anchor cell.
ReferenceWBPaper00026854
TransgeneWBTransgene00031792