WormBase Tree Display for Expr_pattern: Expr1162
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| Expr1162 | Expression_of | Gene | WBGene00004075 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004075 | ||
| Expression_data | Life_stage | WBls:0000006 | |
| WBls:0000007 | |||
| WBls:0000008 | |||
| GO_term | GO:0044444 | ||
| Subcellular_localization | punctate cytoplasmic structures | ||
| Type | Antibody | Rabbit polyclonal antibody to POD-1. | |
| Pattern | POD-1 localization in P cells is cell cycle dependent. In the earliest one-cell embryos before pronuclear meeting (P0), cortical POD-1 is either anteriorly enriched (12/24 embryos), posteriorly enriched (5/24 embryos), or uniformly localized (7/24 embryos). From pronuclear meeting to metaphase, cortical POD-1 is almost always anteriorly enriched (30/39 embryos). At anaphase, POD-1 begins to transition from asymmetric to symmetric localization, such that by telophase cortical POD-1 can be found at equivalent levels in the anterior and posterior cortices (10/11 embryos). In addition to cell cortices, POD-1 antibodies stain punctate cytoplasmic structures. POD-1 can be found in the posterior-most cortex, albeit at lower levels than the anterior. The localization of POD-1 in the germ-line precursors of the two-cell and four-cell embryo is also cell cycle dependent such that early in the cell cycle, POD-1 is not asymmetric along the a-p axis, but becomes anteriorly enriched leading up to metaphase. | ||
| Picture | WBPicture0000008391 | ||
| Remark | To confirm that the localization patterns described are specific to POD-1, pod-1(ye11) mutant embryos were stained with both POD-1 antibodies. All cortical and cytoplasmic POD-1 staining was eliminated in the mutant embryos. | ||
| Reference | WBPaper00003758 | ||
| Antibody_info | WBAntibody00000245 |
