WormBase Tree Display for Expr_pattern: Expr11577
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| Expr11577 | Expression_of | Gene | WBGene00011318 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00011318 | |||
| Expression_data | Life_stage | WBls:0000023 | ||
| Anatomy_term | WBbt:0007809 | Certain | ||
| WBbt:0007831 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0005737 | ||||
| Subcellular_localization | The localisation of CDT-2 fused to GFP is predominantly nuclear in interphase and cytoplasmic during mitosis, which seems contrary with a function in endocytosis. However, the authors cannot exclude that a proportion of CDT-2::GFP below the limit of detection is cytoplasmic during interphase. | |||
| Type | Reporter_gene | |||
| Pattern | CDT-2::GFP is not detected in P cells at larval stage L1, but is expressed early in all Vulval Precursor Cells prior to their first division. The frequency of expression is lowest in P3.p cells, and highest in P6.p. After first division, the cells that adopted the vulval fate all express CDT-2::GFP, but the non-vulval cells generally do not. However, sometimes low expression can be observed in the descendants of P3.p, P4.p and P8.p. Interestingly, after second division (four-cell stage) CDT-2::GFP expression disappears from two secondary cells (P5.ppp and P7.paa); these are the only vulval cells that will not undergo a third cell division. Later, at L4 stage no expression is detected. CDT-2:GFP expression was also observed in the cytoplasm during the first mitotic division of P6.p, which quickly relocalised to the nuclei as the nuclear envelope reforms. The early CDT-2 pattern of expression is consistent with a role during vulval fate adoption, and its down regulation in cells that cease cell division is consistent with a role in DNA replication. | |||
| Picture | WBPicture0000013398 | |||
| Remark | Temporal description | |||
| Reference | WBPaper00037735 | |||
| Transgene | WBTransgene00019627 |
