WormBase Tree Display for Expr_pattern: Expr10609
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| Expr10609 | Expression_of | Gene | WBGene00006975 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006975 | |||
| Expression_data | Life_stage | WBls:0000003 | ||
| WBls:0000041 | ||||
| WBls:0000023 | ||||
| Anatomy_term | WBbt:0005784 | Certain | ||
| WBbt:0006797 | Certain | |||
| GO_term | GO:0005634 | |||
| GO:0000793 | ||||
| Subcellular_localization | Consistent with immunostaining, ZFP-1::GFP localization is seen at the condensed chromosomes in oocytes; the ZFP-1::GFP protein binds to all six condensed chromosomes. The authors conclude that only the long ZFP-1 isoform is expressed in the germline and localizes to chromosomes there. | |||
| Type | Antibody | |||
| Reporter_gene | ||||
| Pattern | ZFP-1::GFP was expressed in somatic cells at all developmental stages of C. elegans. High expression in oocytes and lower expression in the distal germline was observed.Immunostaining showed high expression in maturing oocyte nuclei and lower expression in the rest of the germline. The long isoform of the protein is expressed in the germline, while the short isoform is not. The immunostaining results are consistent with the expression patterns of the short and long isoforms of ZFP-1 determined by western, where the long isoform is predominantly expressed in embryos and in adult worms with developed germline tissue and the short isoform more abundant at the larval stages. The authors conclude that only the long ZFP-1 isoform is expressed in the germline and localizes to chromosomes there. | |||
| Reference | WBPaper00041863 | |||
| Transgene | WBTransgene00018004 | |||
| Antibody_info | WBAntibody00002277 |
