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WormBase Tree Display for Construct: WBCnstr00042223

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Name Class

WBCnstr00042223Summary[ubh-3::ubh-3::gfp]
Driven_by_geneWBGene00006723
GeneWBGene00006723
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[ubh-3::ubh-3::gfp] translational fusion. The ubh-1::gfp, ubh-2::gfp, and ubh-3::gfp translational reporters were generated by PCR amplification of the genomic fragments from 0.7 kb upstream of ubh-1 to the 3'-end of the ubh-1, ubh-2, or ubh-3 coding region. The primers used were as follows: for ubh-1::gfp, 5'-GGCCAAGCTTCTATGCAACTTCCAAAAAC-3' and 5'-GCGCGGATCCTGATTGACAACTGCAATTGC-3'; for ubh-2::gfp, 5'-GCGCGTCGACCAAGTAAATTCTATGCAAC-3' and 5'-CCCCGTCGACAATCCCAACGAGTGCCAT-3'; and for ubh-3::gfp, 5'-GCGCCTGCAGATTCTGGAACTGAATCTCCG-3' and 5'-CGCGCCTCGAGTTTTCCAACAAGAGCCATAGCAGAG-3'. Each fragment was inserted in the BamHI/HindIII, SalI, or PstI/SalI of the pPD95.77 plasmid.
Used_forTransgene_constructWBTransgene00032488
ReferenceWBPaper00060977