WormBase Tree Display for Construct: WBCnstr00042223
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WBCnstr00042223 | Summary | [ubh-3::ubh-3::gfp] | |
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Driven_by_gene | WBGene00006723 | ||
Gene | WBGene00006723 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [ubh-3::ubh-3::gfp] translational fusion. The ubh-1::gfp, ubh-2::gfp, and ubh-3::gfp translational reporters were generated by PCR amplification of the genomic fragments from 0.7 kb upstream of ubh-1 to the 3'-end of the ubh-1, ubh-2, or ubh-3 coding region. The primers used were as follows: for ubh-1::gfp, 5'-GGCCAAGCTTCTATGCAACTTCCAAAAAC-3' and 5'-GCGCGGATCCTGATTGACAACTGCAATTGC-3'; for ubh-2::gfp, 5'-GCGCGTCGACCAAGTAAATTCTATGCAAC-3' and 5'-CCCCGTCGACAATCCCAACGAGTGCCAT-3'; and for ubh-3::gfp, 5'-GCGCCTGCAGATTCTGGAACTGAATCTCCG-3' and 5'-CGCGCCTCGAGTTTTCCAACAAGAGCCATAGCAGAG-3'. Each fragment was inserted in the BamHI/HindIII, SalI, or PstI/SalI of the pPD95.77 plasmid. | ||
Used_for | Transgene_construct | WBTransgene00032488 | |
Reference | WBPaper00060977 |