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WormBase Tree Display for Construct: WBCnstr00041303

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Name Class

WBCnstr00041303Summary[F01D4.5p::GFP]
Driven_by_geneWBGene00008488
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summary[F01D4.5p::GFP] transcriptional fusion: A 2 kb fragment containing the F01D4.5 promoter was generated via PCR. This PCR fragment included the promoter region through the first 5 codons of the first exon of F01D4.5b so as to contain the promoter regions for both isoforms of the gene. This fragment was cloned into the pPD95.75 vector. The resulting F01D4.5p::GFP reporter was modified by homologous recombination to express the unc-119 marker gene as previously described (142). This plasmid was used to bombard HT1593 (unc- 119(ed3)) worms using a previously published protocol (143).
Used_forTransgene_constructWBTransgene00032339
ReferenceWBPaper00056718