WormBase Tree Display for Construct: WBCnstr00023043
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| WBCnstr00023043 | Summary | Pfic-1::FIC-1 E274G | |
|---|---|---|---|
| Driven_by_gene | WBGene00014004 | ||
| Gene | WBGene00014004 | ||
| Other_reporter | Constitutively active AMPylase | ||
| Construction_summary | Pfic-1::FIC-1 E274G was built in a two step cloning process. First, a 1.6 kBps PCR fragment spanning the putative fic-1 promoter was used to replace the eat-4 promoter sequence in pNB7. Next, the fic-1 gene was amplified from C. elegans total cDNA and cloned into the new vector. Point mutations in fic-1 were introduced by SOEing PCR with the mutations encoded in the respective primers. Constitutive fic AMPylase FIC-1(E274G) under the control of the endogenous fic-1 promoter. | ||
| Used_for | Transgene_construct | WBTransgene00023120 | |
| WBTransgene00023130 | |||
| Reference | WBPaper00049562 |
