WormBase Tree Display for Construct: WBCnstr00022633
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| WBCnstr00022633 | Public_name | pMH929 | |
|---|---|---|---|
| Summary | [sur-5p::ARGK-1::GFP] | ||
| Driven_by_gene | WBGene00006351 | ||
| Gene | WBGene00009706 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | DNA constructs for transgenesis were made using the Gateway Technology protocol (Hartley et al.,2000). For argk-1 constructs, PCR products were generated for the C. elegans argk-1/F44G3.2 promoter sequence using primers: Fwd 5' GGG GAC AAC TTT GTA TAG AAA AGT TGA ACT ATT TTT CCC TTCGTG TTT 3' and Rvs 5' GGG GAC TGC TTT TTT GTA CAA ACT TGT CAT GCC AAG ACA CAG AGTTGA 3' (1.2 kb) and for argk-1 cDNA using primers: Fwd 5' GGG GAC AAG TTT GTA CAA AAA AGCAGG CTT GTC ATC TCA AAT TCG CGG CT 3' and Rvs 5' GGG GAC CAC TTT GTA CAA GAA AGCTGG GTA TGT TTC TTT CTC CTC ATC TC 3' (1.1 kb). These PCR products were sequenced and introduced into Gateway entry vectors, which were used to generate the translational reporter pMH929/sur-5p::argk-1::gfp (sur-5 promoter Gateway entry clone was a kind gift from Dr. Supriya Srinivasan). | ||
| Used_for | Transgene_construct | WBTransgene00022877 | |
| Reference | WBPaper00049290 |
