WormBase Tree Display for Construct: WBCnstr00022631
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| WBCnstr00022631 | Summary | [Pgld-1::GEI-12::GFP::3xFLAG::3'unc-54] | |
|---|---|---|---|
| Driven_by_gene | WBGene00001595 | ||
| Gene | WBGene00001569 | ||
| Fusion_reporter | GFP | ||
| Purification_tag | FLAG | ||
| Construction_summary | Full-length gei-12 was amplified from N2 cDNA using the GATEWAY forward primer 5'-GGGGACAAGTTTGTACAAAAAAGCAGGCTTGAGTTCCTCAAAACCTTACCCA-3' and reverse primer 5'-GGGGACCACTTTGTACAAGAAAGCTGGGTATTGATCTCTGGGTGGGTCAAAAATAG-3, cloned into the pDONR221 vector and subcloned into pCFJ150 vector for microparticle bombardment and into a pDEST_EGFP destination vector (gift from Markus Landthaler) for mammalian cell expression. | ||
| Used_for | Transgene_construct | WBTransgene00022875 | |
| Reference | WBPaper00049305 |
