WormBase Tree Display for Construct: WBCnstr00020487
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| WBCnstr00020487 | Summary | [Ppac-1::mCh-pac-1] | |
|---|---|---|---|
| Driven_by_gene | WBGene00015418 | ||
| Gene | WBGene00015418 | ||
| Fusion_reporter | mCherry | ||
| Construction_summary | Ppac-1::mCherry-pac-1 was created by recombineering fosmid WRM063aE11, using SW102 cells and galK selection as described previously. mCherry containing introns was inserted immediately after the start codon of full-length pac-1. Sequence 4,061 bp upstream of the start codon and 554 bp downstream of the stop codon were recombined into plasmid pPUB by gap repair. | ||
| Clone | WRM063aE11 | ||
| Used_for | Transgene_construct | WBTransgene00020913 | |
| Reference | WBPaper00046751 |
