WormBase Tree Display for Construct: WBCnstr00020172
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| WBCnstr00020172 | Public_name | pAG4 | |
|---|---|---|---|
| Summary | [Pnhr-49::nhr-49::gfp] | ||
| Driven_by_gene | WBGene00003639 | ||
| Gene | WBGene00003639 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | To generate the Pnhr-49::nhr-49::gfp construct, 6.6 kb region of nhr-49 gene (4.4 kb comprising the coding region covering all nhr-49 transcripts and 2.2 kb sequence upstream of the first nhr-49 exon) was amplified with primers modified to introduce PstI and SalI restriction sites (forward 5' gctagCTGCAGgaccagaaa- gagcaagagccaatattct 3'; reverse 5' taagcaCCCGGGtcgagcatatgat- tattctgctcactg 3'). The amplified product was cloned into the GFP expression vector pPD95.77 (Addgene plasmid 1495). The full-length nhr-49 fragment was inserted upstream of, and in frame with, GFP at the PstI and SalI sites (pAG4). | ||
| Clone | pPD95.77 | ||
| Used_for | Transgene_construct | WBTransgene00020552 | |
| Interactor | WBInteraction000524595 | ||
| Reference | WBPaper00046079 | ||
| Laboratory | AGP |
