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WormBase Tree Display for Construct: WBCnstr00019307

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Name Class

WBCnstr00019307Other_nameExpr11668_Ex
Summary[Pbas-1::bas-1::gfp]
Driven_by_geneWBGene00000239
GeneWBGene00000239
Fusion_reporterGFP
Type_of_constructCterminal_translational_fusion
Construction_summaryPbas-1::bas-1::gfp transgenic plasmid was generated by joining 4.6 kb bas-1 upstream promoter and full-length bas-1 DNA with GFP reporter gene in the pPD95.75 vector. The bas-1 promoter and bas-1 gene were amplified by PCR from genomic DNA of wild-type N2 worm by the following primers: forward, 5'-acatgcatgcgacaacgaattcgctcattc-3' and reverse, 5'-acccccgggtataccgaactactactgaaag-3' for bas-1 promoter; and forward, 5'-acccccgggatggactcccaaaaattgcg-3' and reverse, 5'-gaactcgccgagaaattattcctaccggtgc-3' for bas-1 gene.
ClonepPD95.75
Used_forTransgene_constructWBTransgene00019982
ReferenceWBPaper00044984
LaboratorySQC