WormBase Tree Display for Construct: WBCnstr00019307
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WBCnstr00019307 | Other_name | Expr11668_Ex | |
---|---|---|---|
Summary | [Pbas-1::bas-1::gfp] | ||
Driven_by_gene | WBGene00000239 | ||
Gene | WBGene00000239 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Cterminal_translational_fusion | ||
Construction_summary | Pbas-1::bas-1::gfp transgenic plasmid was generated by joining 4.6 kb bas-1 upstream promoter and full-length bas-1 DNA with GFP reporter gene in the pPD95.75 vector. The bas-1 promoter and bas-1 gene were amplified by PCR from genomic DNA of wild-type N2 worm by the following primers: forward, 5'-acatgcatgcgacaacgaattcgctcattc-3' and reverse, 5'-acccccgggtataccgaactactactgaaag-3' for bas-1 promoter; and forward, 5'-acccccgggatggactcccaaaaattgcg-3' and reverse, 5'-gaactcgccgagaaattattcctaccggtgc-3' for bas-1 gene. | ||
Clone | pPD95.75 | ||
Used_for | Transgene_construct | WBTransgene00019982 | |
Reference | WBPaper00044984 | ||
Laboratory | SQC |