WormBase Tree Display for Construct: WBCnstr00018160
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WBCnstr00018160 | Other_name | Expr11090_Ex | |
---|---|---|---|
Summary | [LIN-40::GFP; unc-119(+)] | ||
Gene | WBGene00003025 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | The coding sequence of the gfp reporter gene (Chalfie et al., 1994) was excised from the plasmid pPD102.33 (a gift from A. Fire, S. Xu, J. Ahnn and G. Seydoux) and was inserted in-frame after the first methionine residue of the predicted lin-40-coding sequence at a SacI site. Three different GFP reporter constructs were generated. One of them contains the same genomic sequence as in pZC78. In the other two reporter constructs, the genomic sequence after intron II was replaced with either the lin-40a or lin-40b cDNA sequence. | ||
Used_for | Transgene_construct | WBTransgene00018799 | |
Reference | WBPaper00005022 |