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WormBase Tree Display for Construct: WBCnstr00018043

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Name Class

WBCnstr00018043Other_nameExpr11130_Ex
Summary[Pubh-4::GFP::ubh-4 3' UTR]
Driven_by_geneWBGene00006724
UTR_3WBGene00006724
Fusion_reporterGFP
Construction_summaryThis expression vector was created by amplifying 1375 bp of the promoter region upstream and 264 bp downstream of the ubh-4 coding region using PCR. The PCR product amplified upstream of ubh-4 coding region was digested with PciI and AgeI, and the downstream amplified PCR product was digested with NotI. Sequences were cloned into the pPD30.38 expression vector containing GFP coding sequence cloned between inserted AgeI and NotI restriction sites (the unc-54 promoter and enhancer were replaced). --precise ends.
Used_forTransgene_constructWBTransgene00018681
InteractorWBInteraction000520389
ReferenceWBPaper00042588