WormBase Tree Display for Construct: WBCnstr00018043
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WBCnstr00018043 | Other_name | Expr11130_Ex | |
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Summary | [Pubh-4::GFP::ubh-4 3' UTR] | ||
Driven_by_gene | WBGene00006724 | ||
UTR_3 | WBGene00006724 | ||
Fusion_reporter | GFP | ||
Construction_summary | This expression vector was created by amplifying 1375 bp of the promoter region upstream and 264 bp downstream of the ubh-4 coding region using PCR. The PCR product amplified upstream of ubh-4 coding region was digested with PciI and AgeI, and the downstream amplified PCR product was digested with NotI. Sequences were cloned into the pPD30.38 expression vector containing GFP coding sequence cloned between inserted AgeI and NotI restriction sites (the unc-54 promoter and enhancer were replaced). --precise ends. | ||
Used_for | Transgene_construct | WBTransgene00018681 | |
Interactor | WBInteraction000520389 | ||
Reference | WBPaper00042588 |