WormBase Tree Display for Construct: WBCnstr00015758
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WBCnstr00015758 | Summary | [sid-5p::sid-5::GFP] | |
---|---|---|---|
Driven_by_gene | WBGene00017445 | ||
Gene | WBGene00017445 | ||
UTR_3 | WBGene00006789 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [sid-5p::sid-5::GFP] translational fusion. The translational sid-5p::sid-5::GFP fusion was constructed by amplifying the sid-5 promoter and coding region from genomic DNA using primers F14B8afor and F14B8.2ATT- GFPstart and the GFP coding region and unc-54 3'UTR from plasmid pPD95.75 using primers GFP start and GFP reverse unc-54 UTR. The fragments were then joined by PCR stitching using nested primers F14B8jfor and unc-54 reverse nested. | ||
Clone | pPD95.75 | ||
Used_for | Transgene_construct | WBTransgene00016193 | |
Reference | WBPaper00041563 |