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WormBase Tree Display for Construct: WBCnstr00015758

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Name Class

WBCnstr00015758Summary[sid-5p::sid-5::GFP]
Driven_by_geneWBGene00017445
GeneWBGene00017445
UTR_3WBGene00006789
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[sid-5p::sid-5::GFP] translational fusion. The translational sid-5p::sid-5::GFP fusion was constructed by amplifying the sid-5 promoter and coding region from genomic DNA using primers F14B8afor and F14B8.2ATT- GFPstart and the GFP coding region and unc-54 3'UTR from plasmid pPD95.75 using primers GFP start and GFP reverse unc-54 UTR. The fragments were then joined by PCR stitching using nested primers F14B8jfor and unc-54 reverse nested.
ClonepPD95.75
Used_forTransgene_constructWBTransgene00016193
ReferenceWBPaper00041563