WormBase Tree Display for Construct: WBCnstr00013661
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WBCnstr00013661 | Other_name | Expr8993_Ex | |
---|---|---|---|
Summary | [sup-35p::SUP-35-GFP] | ||
Driven_by_gene | WBGene00012976 | ||
Gene | WBGene00012976 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [sup-35p::SUP-35-GFP] translational fusion with full-length SUP-35 fused to GFP under the control of the native sup-35 promoter/enhancer region. A SUP-35::GFP fusion (pDF101)was constructed as follows. An ~2.5-kb sup-35 genomic fragment, which includes the upstream sup-35 promoter/enhancer region, was amplified using the primer pair 5'-GCTCTAGATGATAGTCGTGTCGGTGGTCGTC-3' and 5'-CGCGGATCCAATTGAGCACAAGTCAAGGGCGTCG-3'. This fragment was digested with BamHI and XbaI and cloned in-frame into a similarly restricted pPD95.77 vector. --precise ends. | ||
Clone | pPD95.77 | ||
Used_for | Transgene_construct | WBTransgene00030849 | |
Reference | WBPaper00033464 |