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WormBase Tree Display for Construct: WBCnstr00013661

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Name Class

WBCnstr00013661Other_nameExpr8993_Ex
Summary[sup-35p::SUP-35-GFP]
Driven_by_geneWBGene00012976
GeneWBGene00012976
Fusion_reporterGFP
Type_of_constructTranslational_fusion
Construction_summary[sup-35p::SUP-35-GFP] translational fusion with full-length SUP-35 fused to GFP under the control of the native sup-35 promoter/enhancer region. A SUP-35::GFP fusion (pDF101)was constructed as follows. An ~2.5-kb sup-35 genomic fragment, which includes the upstream sup-35 promoter/enhancer region, was amplified using the primer pair 5'-GCTCTAGATGATAGTCGTGTCGGTGGTCGTC-3' and 5'-CGCGGATCCAATTGAGCACAAGTCAAGGGCGTCG-3'. This fragment was digested with BamHI and XbaI and cloned in-frame into a similarly restricted pPD95.77 vector. --precise ends.
ClonepPD95.77
Used_forTransgene_constructWBTransgene00030849
ReferenceWBPaper00033464