WormBase Tree Display for Construct: WBCnstr00013645
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| WBCnstr00013645 | Other_name | Expr8971_Ex | |
|---|---|---|---|
| Summary | [gem-1::gfp] | ||
| Driven_by_gene | WBGene00008214 | ||
| Gene | WBGene00008214 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [gem-1::gfp] translational fusion constructs. pEJ44: A gem-1:gfp translational fusion generated by inserting the following three segments into pPD95.67 to fuse gfp to the 3'-end of gem-1: (i) a 6.2-kb genomic BamHI fragment that includes the promoter, first exon, first intron, and beginning of the second exon of gem-1; (ii) a segment of gem-1 cDNA corresponding to the remaining portion of exon 2, exon 3, plus the first part of exon 4; and (iii) a genomic segment encompassing the rest of exon 4 through the end of the coding portion of exon 9. pEJ52: Derived from pEJ44 by cutting with BamHI and HpaI and religating with an adapter segment created by annealing oligos o839 gatccttggaagcggccgc and o840 gcggccgcttccaag. This results in the removal of the entire gem-1-coding sequence and creates a unique NotI site at the junction between the first intron of gem-1 and gfp. | ||
| Clone | pPD95.67 | ||
| Used_for | Transgene_construct | WBTransgene00030835 | |
| Reference | WBPaper00032439 |
