WormBase Tree Display for Construct: WBCnstr00011583
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WBCnstr00011583 | Other_name | Expr3665_Ex | |
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Summary | [npp-1::W08F4.8-gfp] | ||
Driven_by_gene | WBGene00003787 | ||
Gene | WBGene00021097 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Translational_fusion | ||
Construction_summary | [npp-1::W08F4.8-gfp] translational fusion.|[pie-1::gfp-W08F4.8] translational fusion. The sequence between the predicted initiation and termination codons, including the intervening introns, was amplified using genomic DNA as a template and primers containing appropriate restriction enzyme sites. These were subsequently cloned into GFP vectors driven by two different promoters: a pie-1 promoter vector, which drives expression of N-terminal GFP fusion protein in the germline (pie-1::GFP::in-ORF), and an npp-1 promoter vector, which drives expression of a C-terminal GFP fusion protein in the germline and the soma (npp-1::in-ORF::GFP). | ||
Used_for | Transgene_construct | WBTransgene00028800 | |
Reference | WBPaper00026722 |