WormBase Tree Display for Construct: WBCnstr00011080
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| WBCnstr00011080 | Other_name | Expr3025_Ex | |
|---|---|---|---|
| Summary | [iri-1::gfp] | ||
| Driven_by_gene | WBGene00002148 | ||
| Gene | WBGene00002148 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [iri-1::gfp] translational fusion. A GFP reporter plasmid was constructed in pHAB200 (a derivative of pPD117.01). A PCR fragment of genomic DNA extending from 4 kb upstream to exon 2 of iri-1 was translationally fused upstream of GFP, while a fragment extending from the stop codon to 2 kb downstream of iri-1 was inserted downstream of GFP. The resulting plasmid was microinjected into C. elegans with the rol-6 marker plasmid pRF4, and stable lines were examined for GFP expression pattern using a Leica SP laser scanning confocal microscope. | ||
| Clone | pRF4 | ||
| Used_for | Transgene_construct | WBTransgene00028306 | |
| Reference | WBPaper00024213 |
