WormBase Tree Display for Construct: WBCnstr00010562
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| WBCnstr00010562 | Other_name | Expr1990_Ex | |
|---|---|---|---|
| Summary | [cyp-5::lacZ] | ||
| Driven_by_gene | WBGene00000881 | ||
| Gene | WBGene00000881 | ||
| Fusion_reporter | LacZ | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [cyp-5::lacZ] translational fusion. The potential upstream regulatory region of cyp-5 was predicted from cosmid F31C3. The 2674-base-pair 50 untranslated region of the cyp-5 gene was amplified from genomic DNA with Taq polymerase (AB Gene). The PCR primers contained an artificial restriction site (lowercase and underlined) to allow directional subcloning into the multiple cloning sites of the vector pPD95.03 and allow an in-frame translational fusion to the lacZ gene with the first two amino acid residues of CYP-5; cyp5pF 50-gctctagagTGGAGTTGCGAGCGAAAATCATG-30 (sense, Xba I); cyp5pR 50-gcggatccTTCATACCTGCAAAAATTATGGTG-30 (antisense, Bam HI). | ||
| Clone | pPD95.03 | ||
| Used_for | Transgene_construct | WBTransgene00027794 | |
| Reference | WBPaper00005482 |
