WormBase Tree Display for Construct: WBCnstr00010315
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| WBCnstr00010315 | Public_name | pMT1.1 | |
|---|---|---|---|
| Other_name | Expr1508_Ex | ||
| Summary | [mtl-1::lacZ] | ||
| Driven_by_gene | WBGene00003473 | ||
| Fusion_reporter | LacZ | ||
| Construction_summary | This fusion construct was generated by using a 1.7-kbp fragment of the mtl-1 gene that is immediately upstream from the initiator ATG. The 5' primer AF-80 corresponds to nt170-193 in pGEM7z and precedes the EcoR I site present in the polylinker of the vector. The 3' primer (AF-136) includes the reverse complement of nt 1-20 in CeMTl cDNA and incorporates an XbaI site at the 3'end of the amplified DNA. The amplified promoter fragment was digested with EcoRI and XbaI and then cloned into a C. elegans expression plasmid as previously described. In the resulting construct, a DNA segment that encodes beta-galactosidase preceded by the nuclear targeting sequence is positioned immediately downstream from the sequence. | ||
| Used_for | Transgene_construct | WBTransgene00027554 | |
| Reference | WBPaper00001688 |
