WormBase Tree Display for Construct: WBCnstr00006909
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WBCnstr00006909 | Public_name | pHD429 | |
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Summary | [Pelt-2::mCherry:CUP-5] | ||
Driven_by_gene | WBGene00001250 | ||
Gene | WBGene00000846 | ||
Fusion_reporter | mCherry | ||
Construction_summary | This construct was made by inserting the elt-2 promoter into mCherry::CUP-5 plasmid pHD260. The elt-2 promoter was PCR amplified from pJM260, and restriction digested with PstI and BstUI. pHD260, was restriction digested with BmtI, incubated with T4 DNA Polymerase to create a blunt end, and then digested with PstI. The promoter fragment (5057 bp) was then ligated to the pHD260 fragment (6227 bp) to make pHD429, used to make transgenic lines (Campbell et al., 2010). | ||
Used_for | Transgene_construct | WBTransgene00007084 | |
Reference | WBPaper00036378 |