WormBase Tree Display for Construct: WBCnstr00006715
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| WBCnstr00006715 | Public_name | pK7GL44.2 | |
|---|---|---|---|
| Summary | [let-23::GFP] | ||
| Driven_by_gene | WBGene00002299 | ||
| WBGene00001079 | |||
| Gene | WBGene00002299 | ||
| WBGene00001079 | |||
| Fusion_reporter | GFP | ||
| Construction_summary | Plasmid pk7GL44.2, containing the let-23::GFP fusion, was constructed as follows: site-directed mutagenesis of let-23 genomic DNA was carried out, as described previously, in pk7-5.5, a HindIII clone of let-23 that contains the last 3 kb of a 3' coding sequence plus ~2 kb of 3' untranslated sequence, so that the let-23 stop codon and surrounding sequences were changed to an SphI site. At this site, a S65T GFP cassette amplified by PCR with primers containing the SphI site was cloned. This fragment was then excised with SalI and HindIII and ligated with an ~12 kb EcoRI-SalI genomic let-23 fragment and with an 3 kb EcoRI-HindIII pBluescript II fragment generating the plasmid pk7GL44.2 (Chang et al., 1999). | This construct contains 3.4 kb of sequence upstream of the let-23 start ATG. (Van Buskirk and Sternberg, 2010). | ||
| Used_for | Transgene_construct | WBTransgene00006878 | |
| Reference | WBPaper00003429 | ||
| WBPaper00028466 | |||
| WBPaper00028559 | |||
| WBPaper00036308 | |||
| WBPaper00038525 |
