WormBase Tree Display for Construct: WBCnstr00006526
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| WBCnstr00006526 | Public_name | pNL212 | |
|---|---|---|---|
| Summary | [myo-3p::GFP::DAF-16] | ||
| Driven_by_gene | WBGene00003515 | ||
| Gene | WBGene00000912 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | To generate this daf-16 tissue-specific promoter fusion, HindIII and SnaB1 sites were introduced immediately 5' to the first ATG by PCR amplification of the daf-16 cDNA construct (yk13f11, identified by Y. Kohara) using an inverse PCR strategy (HindIII is more proximal to the ATG). A HindIII GFP fragment from the L2911 plasmid (gift from A. Fire) was inserted into the HindIII site to generate an in-frame fusion of GFP immediately upstream of the N terminus of DAF-16a (pNL205). The SnaB1 site was used to insert the muscle Pmyo-3 promoter, which has 2.5 kb upstream regulatory sequence of this gene PCR-amplified from pPD93.97 (gift from A. Fire) (pNL212) (Libina et al. 2003). | ||
| Used_for | Transgene_construct | WBTransgene00006683 | |
| Reference | WBPaper00006226 | ||
| WBPaper00035871 | |||
| WBPaper00042119 |
