WormBase Tree Display for Construct: WBCnstr00006287
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| WBCnstr00006287 | Summary | [Ptrp-4::ICE, Pelt-2::gfp] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006616 | ||
| WBGene00001250 | |||
| Fusion_reporter | GFP | ||
| Other_reporter | ICE | ||
| Construction_summary | This transgene killes the RnA neuron. Initially, the P(trp- 4)::gfp plasmid EM#316 was generated by inserting the 8kb region upstream of the trp-4 gene (from 25692 to 33658 of Y71A12B) into the pPD95.75 vector. The P(trp-4)::ICE construct EM#319 was generated by removing the gfp coding sequence from EM#316 and replacing it by the ICE coding region from the P(dat-1)::ICE plasmid (SalI-ApaI region in both plasmids). bxEx138 transgenic worms were generated by coinjecting EM#319 at 20 ng/l with the marker elt-2::gfp (50 ng/l) into bxIs19 (Barrios et al., 2008) . | ||
| Used_for | Transgene_construct | WBTransgene00006440 | |
| Reference | WBPaper00032414 |
