WormBase Tree Display for Construct: WBCnstr00000527
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| WBCnstr00000527 | Public_name | pDE218 | |
|---|---|---|---|
| Other_name | Expr1395_Ex | ||
| Summary | [bar-1::gfp] | ||
| Driven_by_gene | WBGene00000238 | ||
| Gene | WBGene00000238 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | The bar-1::GFP reporter plasmid pDE218 was constructed by ligating together: (1) a 6.3 kb PstI-ClaI fragment containing bar-1 upstream DNA (from the BglII site of pDE204 to the ClaI site in the open reading frame), (2) a 3.1 kb ClaI-BamHI fragment containing bar-1 genomic DNA encoding residues 1-761, and (3) the GFP reporter vector pPD95.75 digested with PstI and BamHI. The resulting plasmid rescues the bar-1 mutant phenotypes. | ||
| Used_for | Transgene_construct | WBTransgene00000528 | |
| WBTransgene00020335 | |||
| Reference | WBPaper00003200 | ||
| WBPaper00032129 | |||
| WBPaper00032331 |
