WormBase Tree Display for Clone: RST5_519918
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RST5_519918 | Species | Caenorhabditis elegans | |
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Sequences | Sequence | RST5_519918 | |
Remark | General_remark | This is a unpublished clone from the Orfeome Cloning Project RACE sub-project | |
To generate the RACE fragments, we reverse transcribed total C. elegans RNA (isolated from mix-stage, asynchronously growing N2 worm population) using either dT16 (for 5 Prime RACE) or used our tailed dT primer (for 3 Prime RACE). Nested PCRs were performed to increase sensitivity and specificity. The generated PCR products were then cloned recombinationally and sequenced from the 5 Prime end, generating RACE Sequence Tags (or RSTs). | |||
Type | cDNA |