WormBase Tree Display for Antibody: WBAntibody00002769
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| WBAntibody00002769 | Summary | A C-terminal fragment consisting of amino acids 1,350-1,470 from recombinant C. elegans topo-II was amplified with the primers 5'-GCG CGCCCATGGATGTTCGATTCGGACGATGAC-3' and 5'-GCGCGC CTCGAGTCATCTTGGAGCCACAACGAAGC-3'. This fragment was inserted into a bacterial expression vector (pGEX; GE Life Sciences) and expressed in Escherichia coli cells. Purified protein was used for antibody production in rabbit (Institute for Research in Immunology and Cancer core facility). Topo-II antibodies were immunopurified using the same fragment fused to a his tag using standard technologies. A fraction of topo-II and CENP-A antibodies was labeled with DyLight 499 and 649 (DyLight Microscale Labeling kit; Thermo Fisher Scientific). Antibodies were used at concentrations of 10 and 0.33 μg/ml for immunofluorescence and Western blotting, respectively. | ||
|---|---|---|---|---|
| Public_name | [WBPaper00051533]::anti-TOPO-2 | |||
| Gene | WBGene00010785 | |||
| Isolation | Original_publication | WBPaper00051533 | ||
| Clonality | Polyclonal | |||
| Antigen | Protein | Amino acids 1,350-1,470 | ||
| Animal | Rabbit | |||
| Reference | WBPaper00051533 | |||
| WBPaper00064630 |
