WormBase Tree Display for Analysis: Microarray_Study.WBPaper00064957.ce.mr
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| Microarray_Study.WBPaper00064957.ce.mr | DB_info | Database | GEO | GSE201599 |
|---|---|---|---|---|
| Title | Transcriptomic Analysis Reveals JAK2/MPL-Independent Effects of Calreticulin Mutations in a C. elegans Model. | |||
| Description | There is growing evidence that Ph-negative myeloproliferative neoplasms (MPNs) are blood cancers in which multiple molecular mechanisms are significantly disturbed. Since their discovery in 2016, CALR (calreticulin) type 1 and type 2 driver mutations have been demonstrated to trigger pathogenic mechanisms apart from the well-documented activation of JAK2/MPL-related pathways affecting these diseases. C. elegans seems to be a suitable model for the study of these mechanisms since they have a CALR ortholog (crt-1) but have no JAK or MPL ortholog. We used microarrays in C. elegans strains harboring patient-like calreticulin mutations to identify JAK/MPL-independent molecular mechanisms derived from mutant calreticulin and identified a significant disruption of some of the typically altered biological processes in cancer and Ph-negative MPN patients. Finally, we compared the expression of the genes participating in these processes with a calreticulin knockout strain in order to examine if the alteration of these processes is a consequence of a gain or a loss of function of the mutant protein. | |||
| Category | WT vs. mutant | |||
| Microarray_experiment | WBPaper00064957:crt-1(knu358)_rep1 | |||
| WBPaper00064957:crt-1(knu358)_rep2 | ||||
| WBPaper00064957:crt-1(jvp1)_rep1 | ||||
| WBPaper00064957:crt-1(jvp1)_rep2 | ||||
| WBPaper00064957:crt-1(jvp1)_rep3 | ||||
| WBPaper00064957:N2_rep1 | ||||
| WBPaper00064957:N2_rep2 | ||||
| WBPaper00064957:N2_rep3 | ||||
| WBPaper00064957:crt-1(jh101)_rep1 | ||||
| WBPaper00064957:crt-1(jh101)_rep2 | ||||
| Species_in_analysis | Caenorhabditis elegans | |||
| Reference | WBPaper00064957 |
