WormBase Tree Display for Variation: WBVar00091509

WBVar00091509 Evidence: Paper_evidence: WBPaper00039866
  Name: Public_name: ok200
    HGVSg: CHROMOSOME_IV:g.11800544_11801794del
  Sequence_details: SMap: S_parent: Sequence: H01G02
    Flanking_sequences: aaactcacttttgaaacattcgggaccatt gatgaagatcatggaacgttgcaatcaatt
    Mapping_target: H01G02
    Type_of_mutation: Deletion
    PCR_product: OK200_external
      OK200_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00007240
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00045108
      WBGene00010349
    Transcript: H01G02.4.2 VEP_consequence: splice_acceptor_variant,splice_donor_variant,3_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: 886-?
        Intron_number: 6/6
        Exon_number: 6-7/7
      H01G02.4.1 VEP_consequence: 3_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: 886-?
        Exon_number: 6/6
      H01G02.2.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-206
        CDS_position: ?-116
        Protein_position: ?-39
        Intron_number: 2/8
        Exon_number: 1-3/9
    Interactor: WBInteraction000563598
      WBInteraction000579025
      WBInteraction000579026
  Isolation: Mutagen: UV/TMP
  Description: Phenotype: WBPhenotype:0000249 Paper_evidence: WBPaper00039866
        Curator_confirmed: WBPerson712
        Remark: The strain was impaired in its ability to avoid substances of high osmolarity (Osm phenotype). Paper_evidence: WBPaper00039866
            Curator_confirmed: WBPerson712
      WBPhenotype:0000255 Paper_evidence: WBPaper00054177
        Curator_confirmed: WBPerson22262
        Remark: suppression of dye filling defective Paper_evidence: WBPaper00054177
            Curator_confirmed: WBPerson22262
        EQ_annotations: Anatomy_term: WBbt:0005391 PATO:0000460 Paper_evidence: WBPaper00054177
                Curator_confirmed: WBPerson22262
            WBbt:0005425 PATO:0000460 Paper_evidence: WBPaper00054177
                Curator_confirmed: WBPerson22262
      WBPhenotype:0000436 Paper_evidence: WBPaper00039866
        Curator_confirmed: WBPerson712
        Remark: The dyf-18 (ok200) mutant showed prominent accumulations of the homodimeric kinesin-II motor, OSM-3, between the middle and the distal segment both in the amphid and phasmid neuron cilia in 100% of the mutant worms. The IFT protein OSM-5, on the other hand, was frequently observed to accumulate at the base of cilia in amphid and phasmid neurons, with much reduced localization along the ciliary axoneme. Paper_evidence: WBPaper00039866
            Curator_confirmed: WBPerson712
      WBPhenotype:0000615 Paper_evidence: WBPaper00039866
          WBPaper00056552
        Curator_confirmed: WBPerson712
          WBPerson35872
        Remark: The structure of most cilia appeared superficially similar to wild type, with occasionally long curved cilia. Paper_evidence: WBPaper00039866
            Curator_confirmed: WBPerson712
          long and unbranched AWA cilia Paper_evidence: WBPaper00056552
            Curator_confirmed: WBPerson35872
        EQ_annotations: Anatomy_term: WBbt:0005670 PATO:0000460 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
          GO_term: GO:0005929 PATO:0000573 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
              PATO:0000414 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
      WBPhenotype:0000854 Paper_evidence: WBPaper00054177
        Curator_confirmed: WBPerson22262
        EQ_annotations: GO_term: GO:0042073 PATO:0000460 Paper_evidence: WBPaper00054177
                Curator_confirmed: WBPerson22262
      WBPhenotype:0002471 Paper_evidence: WBPaper00056552
        Curator_confirmed: WBPerson35872
        Remark: long and unbranched AWA cilia Paper_evidence: WBPaper00056552
            Curator_confirmed: WBPerson35872
        EQ_annotations: Anatomy_term: WBbt:0005670 PATO:0000460 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
          GO_term: GO:0005929 PATO:0000573 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
              PATO:0000414 Paper_evidence: WBPaper00056552
                Curator_confirmed: WBPerson35872
      WBPhenotype:0002535 Paper_evidence: WBPaper00039866
        Curator_confirmed: WBPerson712
        Remark: Compared to wild-type animals, ok200 mutant animals display only a weak ability to uptake the dye in amphid (head) and phasmid (tail) neurons. Paper_evidence: WBPaper00039866
            Curator_confirmed: WBPerson712
  Reference: WBPaper00039866
    WBPaper00054177
    WBPaper00056552
    WBPaper00065813
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele