WormBase Tree Display for Variation: WBVar00090707
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| WBVar00090707 | Evidence | Paper_evidence | WBPaper00028753 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Public_name | n3423 | |||||||
| Sequence_details | Mapping_target | JC8 | |||||||
| Type_of_mutation | Deletion | ||||||||
| SeqStatus | Pending_curation | ||||||||
| Variation_type | Allele | ||||||||
| Origin | Species | Caenorhabditis elegans | |||||||
| Strain | WBStrain00027517 | ||||||||
| WBStrain00027524 | |||||||||
| Laboratory | MT | ||||||||
| Status | Live | ||||||||
| Affects | Gene | WBGene00003037 | |||||||
| Interactor | WBInteraction000502683 | ||||||||
| WBInteraction000502688 | |||||||||
| Description | Phenotype | WBPhenotype:0000120 | Paper_evidence | WBPaper00038427 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Control experiments showed that wild-type and lin- 54(n2990) mutant animals produce a comparable amount of fulllength, nuclear-localized LIN-54 protein (Figure 2A and 2B), unlike lin-54(n3423) null animals which produce no detectable LIN-54 protein and reduced amounts of other DRM subunits (Figure 2B and [4])." | Paper_evidence | WBPaper00038427 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000504 | Paper_evidence | WBPaper00038427 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "lin-54 mutants also exhibit inappropriately connected gut nuclei (right), which may result from defects in mitotic chromosome segregation." (Figure S3A) | Paper_evidence | WBPaper00038427 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00038427 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000668 | Paper_evidence | WBPaper00038427 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | Figure S3A | Paper_evidence | WBPaper00038427 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0000717 | Paper_evidence | WBPaper00038427 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | Table S2, germline microarray: "In the germline, 78 genes showed increased and 251 genes showed decreased expression in mutant relative to wild-type animals (Figure 4A, Table S2)." | Paper_evidence | WBPaper00038427 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0005784 | PATO:0000460 | Paper_evidence | WBPaper00038427 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_assay | Treatment | "To identify genes regulated by LIN-54 in vivo, we performed microarray expression profiling analysis of wild-type and lin-54 mutant C. elegans embryos and of isolated germlines... Germlines were dissected from lin-54 null adults that lack detectable lin-54 transcript and protein ([4], Figure 2, and data not shown), exhibit reduced levels of other DRM complex proteins [4], and exhibit reduced germline chromosome association of DRM complex proteins tested (Figure S1D). We isolated the germline region from the tip until late pachytene stage of meiosis, because nuclei in this region are morphologically similar between wild-type and mutant (Figure S3B) and are undergoing X chromosome silencing [26]." | Paper_evidence | WBPaper00038427 | |||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Reference | WBPaper00038427 | ||||||||
| WBPaper00028753 | |||||||||
| Method | Deletion_allele |
