WormBase Tree Display for RNAi: WBRNAi00089959

WBRNAi00089959 Homol: Homol_homol: R12H7:RNAi
  Sequence_info: PCR_product: mv_AAB51534
  Experiment: Laboratory: CC
    Date: 15 Aug 2011 00:00:00
    Genotype: ccIs55 [unc-54::lacZ]
    Treatment: 3-4 L4 hermaphrodite worms were placed on an RNAi plate and incubated for 72-96 hours at 20 degrees Celsius. Young adult progeny were then scored for cytosolic protein levels by LacZ staining. An additional examination of cytosolic protein levels was made in adults again 24 hours later. L4 hermaphrodites from the F1 generation were then transferred to newly seeded RNAi plates for examination of the F2 generation. Genes whose knockdown was observed to affect cytosolic protein levels in the F1 and F2 generations above were retested by exposing adult worms to an acute exposure of RNAi bacteria. In these experiments, worms were raised to young adulthood on non-RNAi bacteria at 20 degrees Celsius and then manually transferred to RNAi plates. Cytosolic protein levels were examined 24, 48, and 72 hours post transfer.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: R12H7.1a Inferred_automatically: RNAi_primary
      R12H7.1b Inferred_automatically: RNAi_primary
    Gene: WBGene00006749 Inferred_automatically: RNAi_primary
    Transcript: R12H7.1a.2 Inferred_automatically: RNAi_primary
      R12H7.1a.1 Inferred_automatically: RNAi_primary
      R12H7.1b.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00040284
  Phenotype: WBPhenotype:0001645 Remark: RNAi was observed to significantly reduce cytosolic protein levels in body wall muscles, as determined by reduced LacZ staining compared to controls. This affect was observed after chronic RNAi exposure (RNAi continuously for two generations), but not after acute exposure to RNAi only during adulthood.
  Remark: (Figure 2) unc-9 RNAi
  Method: RNAi