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WormBase Tree Display for RNAi: WBRNAi00080273

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Name Class

WBRNAi00080273HomolHomol_homolC25G4:RNAi
Sequence_infoPCR_productsjj_C25G4.5
ExperimentLaboratoryKIR
Date04 Dec 2008 00:00:00
Genotypehim-8(e1489); xol-1(y9) sex-1(y263)
Treatmenthim-8(e1489); xol-1(y9) sex-1(y263) hermaphrodites were grown from L1 to adulthood on RNAi plates. Two adults were moved to fresh plates and allowed to lay eggs for 24 hours. Total progeny was counted as soon as mothers were removed from the plates. In three days, progeny was scored as either male or hermaphrodite. Male rescue was calculated as # males/expected number of males x 100. The expected number of males was assumed to be 38% of total progeny, due to the presence of the him-8 mutation [10]. At least 200 animals were scored for RNAi of each gene.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC25G4.5Inferred_automaticallyRNAi_primary
GeneWBGene00001085Inferred_automaticallyRNAi_primary
TranscriptC25G4.5.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000051401
ReferenceWBPaper00032450
PhenotypeWBPhenotype:0000050RemarkRNAi of dpy-26 rescues the male-specific embryonic lethality of the sex-1(y263);xol-1(y9) mutation
PenetranceIncomplete
Range33
MethodRNAi