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| WBPicture0000013580 | Description | Figure S1. DamID fusion proteins localize properly at the NE. (A) Schematic representation of Dam-fusion constructs. Chimeric proteins were expressed under the control of the hsp-16.41 heat- shock promoter and the unc-54 3'UTR. A MYC epitope was inserted between the proteins of interest and Dam to facilitate detection of the chimera. Not drawn to scale. (B-D) Embryos were obtained from nematodes incubated constantly at 20oC or heat-shocked 1h at 33oC and left to recover for 2h at 20oC. (B) Western-blot analysis of embryos from wild type (N2) and transgenic strains expressing Dam::EMR-1, Dam::LMN-1 or GFP::Dam fusion proteins probed with anti- MYC antibody. (C) Dam::LMN-1 and Dam::EMR-1 expressing embryos were fixed and stained with anti-MYC antibody (green) and Hoechst 33258 to visualize chromatin (blue). Localization to the NE is observed for both fusion proteins after heat shock. (D) GFP::Dam expressing embryos were analyzed by live confocal microscopy. Arrows point to nuclei, whereas an open triangle indicates GFP::Dam associated with metaphase chromosomes. Boxed regions in merge images (right) are shown at higher magnification to the left. Scale bars, 10um. | |||
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| Name | S1.jpg | ||||
| Crop | Crop_picture | WBPicture0000013581 | |||
| Acknowledgment | Template | From: <Journal_URL> <Article_URL>. Copyright <Publication_year> <Publisher_URL>. | |||
| Publication_year | 2014 | ||||
| Article_URL | DOI | id | 10.1186/gb-2014-15-2-r21 | ||
| Journal_URL | GenomeBiology | ||||
| Publisher_URL | BiomedCentralLTD | ||||
| Reference | WBPaper00044786 |
