WormBase Tree Display for Picture: WBPicture0000011198

WBPicture0000011198 Description: Figure 8. Ontogeny of mRNAs for gpd-4 and gpd-2. 10 g of total embryonic (E) and postembryonic larval (L4) mRNAs were dot blotted on to nitrocellulose paper and probed with gpd-2 and gpd-4-specific probes. The probe for gpd-2 was prepared using pSS1.3 (Fig. 3) derived from an M13 phage DNA using an oligonucleotide primer located 196 bp from its termination codon, which extended to a HaeIII site 5 bp before its stop codon. Since the 5' end of gpd-3 obtains a trans-spliced leader sequence (see Results and Discussion) this probe is specific for gpd-2 only. The gene-specific probe for gpd-4 was prepared using pB9 (Fig. 1(b)), an oligonucleotide 164 bp downstream from its termination codon, which was then extended to a HaeIII site located 10 bp beyond its stop codon.
  Name: FigM.jpg
  Depict: Expr_pattern: Expr777
      Expr778
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
    Publication_year: 1989
    Article_URL: DOI id 10.1016/0022-2836(89)90490-7
    Journal_URL: JournalofMolecularBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00001143