Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Picture: WBPicture0000011197

expand all nodes | collapse all nodes | view schema

Name Class

WBPicture0000011197DescriptionFigure 7. Ontogeny of mRNAs for gpd-3 and gpd-1. Total RNA from the designated embryonic (E), 1st larval (L1), 2nd larval (L2) and 4th larval (L4) stage were purified and separated on agarose as described in Materials and Methods. Poly(A)' RNA was purified from total embryo RNA by oligo(dT)-cellulose chromatography (E+). RNA molecular weight standard markers were obtained from Bethesda Research Laboratories. RNA (10 g) was applied to each lane. (a) Upon transfer to a nitrocellulose filter, the panel of RNAs were hybridized with a probe specific for gpd-3. The gene specific probe for gpd-3 was made by primer extension of pSB (Fig. 3) using an oligonucleotide located 240 bp downstream from its termination codon, which extended to a HaeIII site located 5 bp before its stop codon. (b) A duplicate filter was challenged with a probe specific for gpd-1. The gene-specific probe for gpd-1 was made by primer extension of plasmid pSE3' (Fig. 1(a)) using an oligonucleotide primer 180 bp downstream from its termination codon. The extension was extended to a Sau3A site located 10 bp beyond the termination codon.
NameFigL.jpg
DepictExpr_patternExpr776
Expr779
AcknowledgmentTemplateWormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>.
Publication_year1989
Article_URLDOIid10.1016/0022-2836(89)90490-7
Journal_URLJournalofMolecularBiology
Publisher_URLElsevier
ReferenceWBPaper00001143