WormBase Tree Display for Antibody: WBAntibody00000361
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| WBAntibody00000361 | Summary | Mouse monoclonal antibody against UNC-1 recombinant protein. Antibodies to UNC-1 were prepared in the laboratory of Man Sun Sy. Two mice were inoculated three times with UNC-1 protein. Once a response was documented from the serum of the mice, hybridomas were made and screened for anti-UNC-1. Seven monoclonal antibodies reacting with UNC-1 were isolated. Each antibody was screened for the ability to bind UNC-1 on a Western blot and for immunocytochemical staining in N2, as well as loss of staining in the unc-1 null alleles fc53 and e580. Five monoclonal antibodies specific for UNC-1 were isolated, each of which gave identical binding in nematodes and on Western blots. One monoclonal antibody, 5D5, was used for all the studies. | ||
|---|---|---|---|---|
| Public_name | [cgc4649]:unc-1 | |||
| Gene | WBGene00006741 | |||
| Isolation | Location | CW | ||
| Clonality | Monoclonal | |||
| Antigen | Protein | The cDNA for UNC-1 was subcloned into the multiple cloning site of the pET32A vector using the restriction enzymes BamHI and HindIII (Invitrogen). This vector is one of the series of expression vectors containing six tandem histidine residues in the NH2-terminal peptide that have a high affinity for ProBond resin. The his tag was used to purify the expressed protein using the Xpress System from Invitrogen. | ||
| Animal | Mouse | |||
| Expr_pattern | Expr887 | |||
| Interactor | WBInteraction000556982 | |||
| WBInteraction000556983 | ||||
| WBInteraction000556985 | ||||
| Reference | WBPaper00004649 |
