WormBase Tree Display for Expr_pattern: Expr9851
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| Expr9851 | Expression_of | Gene | WBGene00001833 |
|---|---|---|---|
| WBGene00009254 | |||
| WBGene00001085 | |||
| WBGene00001087 | |||
| Reflects_endogenous_expression_of | WBGene00001085 | ||
| WBGene00001087 | |||
| WBGene00001833 | |||
| WBGene00009254 | |||
| Expression_data | GO_term | GO:0005694 | |
| GO:0005876 | |||
| GO:0072687 | |||
| Subcellular_localization | After nuclear envelope breakdown (NEBD), the two condensin complexes occupied distinct domains. Condensin I was found at the interface between homologs marked by reduced DAPI staining ("DAPI-free zone"), whereas condensin II localizes to sister chromatids throughout meiosis. During anaphase, condensin I localized on the acentrosomal meiotic spindle between separating chromosomes. As in mitosis, condensin I colocalized with AIR-2 on chromosomes during prometaphase and metaphase of meiosis, and on the spindle during anaphase. Condensin I localizes to the short arms of bivalents, where cohesion between the exchanged parts of sister chromatids holds homologs together. | ||
| Condensin localization in the male germline followed the same general pattern. Both condensins I and II began to accumulate in nuclei by late pachytene. Condensin II associated with chromosomes before NEBD, but condensin I chromosomal enrichment was not seen until after NEBD in late diakinesis. Following NEBD, condensin I was between aligned homologs (meiosis I) and between sisters (meiosis II), whereas condensin II remained associated with sister chromatids throughout. Interestingly, condensin I behavior in sperm and oocytes differed at anaphase. In oocytes, condensin I colocalized with microtubules and AIR-2 between separating chromosomes during anaphase. In sperm, AIR-2 and microtubules were not prominent between separating chromosomes, and condensin I was absent from this region. Instead, condensin I and AIR-2 colocalized on the inner edges of separating chromosomes. | |||
| In early prophase, only condensin II associated with chromosomes. Condensin I loaded onto chromosomes after nuclear pore complexes disassembly in prometaphase. From prometaphase to anaphase, the spatial patterns of condensins I and II were different, with condensin II in a centromere-like pattern and condensin I diffusely coating all chromosomes. During anaphase, condensin I was also seen colocalizing with microtubules at the spindle midzone in addition to the chromosomal signal. The other condensin I CAP subunits, DPY-26 and DPY-28, associated with chromosomes at the same time and with similar patterns to that of CAPG-1. Furthermore, the chromosomal association of CAPG-1 was dependent on the presence of DPY-26 and DPY-28, indicating that the condensin I CAP subunits associate with mitotic chromosomes as a complex. | |||
| Type | Antibody | ||
| Reference | WBPaper00040358 | ||
| Antibody_info | WBAntibody00000813 | ||
| WBAntibody00000814 | |||
| WBAntibody00001707 | |||
| WBAntibody00001708 | |||
| WBAntibody00001962 | |||
| WBAntibody00001964 | |||
| WBAntibody00001965 | |||
| WBAntibody00002023 | |||
| WBAntibody00002032 |
