WormBase Tree Display for Expr_pattern: Expr887
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| Expr887 | Expression_of | Gene | WBGene00006741 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00006741 | |||
| Expression_data | Life_stage | WBls:0000021 | ||
| Anatomy_term | WBbt:0005656 | Certain | ||
| WBbt:0005829 | Certain | |||
| WBbt:0006749 | Certain | |||
| WBbt:0006750 | Certain | |||
| GO_term | GO:0030424 | |||
| Subcellular_localization | UNC-1 was not found in the nucleus but, rather, was distributed along the presumed axons of neurons. | |||
| Type | Antibody | anti-UNC-1 antibody, | ||
| Pattern | Staining wild-type C. elegans with anti-UNC-1 antibody showed a punctate distribution of protein along nerve tracts. Staining was most intense in the nerve ring, the retrovesicular ganglion, and the ventral and dorsal nerve cords. Staining was also prominent in the nerve tracts of the snout, around the vulva, and along the sublateral nerve tracts of the body. No staining was seen in other tissues. Staining was noted in late embryos in the nerve ring and ventral nerve cord. | |||
| Remark | Incubation of UNC-1 antibody with fc53, a null allele of unc-1, showed no staining.The staining of N2 was consistent with the fluorescence reported previously in live unc-1(0) animals rescued with a GFP-UNC-1 fusion construct. Antibody staining of the dominant unc-1 allele n494 showed a pattern similar to that for N2; however, the class II allele n774 showed less oveall staining than N2 in a grossly normal distribution. | |||
| The cellular distribution of UNC-1 was localized by co-staining with DAPI, for nuclear staining, and by co-staining with anti-UNC-17 (vesicular acetylcholine transporter, VAChT), a marker of cholinergic synapses. | ||||
| late embryo(author) = fully-elongated embryo(curator) | ||||
| Reference | WBPaper00004649 | |||
| Antibody_info | WBAntibody00000361 |
