WormBase Tree Display for Expr_pattern: Expr3459
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| Expr3459 | Expression_of | Gene | WBGene00002062 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002062 | |||
| Homol | Homol_homol | C05D9:Expr | ||
| Expression_data | Life_stage | WBls:0000024 | ||
| WBls:0000038 | ||||
| WBls:0000027 | ||||
| WBls:0000035 | ||||
| WBls:0000041 | ||||
| WBls:0000021 | ||||
| Anatomy_term | WBbt:0004757 | Certain | ||
| WBbt:0004758 | Certain | |||
| WBbt:0005304 | Certain | |||
| WBbt:0005319 | Certain | |||
| WBbt:0005439 | Certain | |||
| WBbt:0005451 | Certain | |||
| WBbt:0005821 | Certain | |||
| WBbt:0005829 | Certain | |||
| WBbt:0006759 | Certain | |||
| Type | Reporter_gene | [ife-4::gfp] translational fusions. One was a simple array containing an NLS, ife-4::NLS::GFP(lsEx296), and the other, a complex array without an NLS, ife-4::GFP(lsEx385). The array with NLS was used to facilitate the identification of ife-4::GFP-expressing cells, whereas the array without NLS was expected to produce a subcellular distribution more similar to that of native IFE-4. ife-4::GFP was generated by using a PCR-based protocol. Briefly, the complete ife-4 coding sequence and 1.5 kb upstream of the 5$(B!l(B-trans-spliced acceptor site were amplified from genomic DNA (F, 5$(B!l(B-TGAAGCTTTCAATTTTCATTTCCAGGCG-3$(B!l(B; R, 5$(B!l(B-AGCTTGCATGCCTGCAGGTCGACTTTTGCAGATATTT-3$(B!l(B). The reverse primer contained a 24-nucleotide (nt) overlap to the GFP sequence. The GFP coding region, preceded by a nuclear localization signal (NLS) and followed by the unc-54 3$(B!l(B-UTR, was amplified from pPD95.67. Nested primers were used to create DNA encoding a fusion protein in a second PCR round. This DNA was microinjected into the gonad of N2 animals with 50 ng of the marker plasmid pRF4 carrying a dominant rol-6(su1006) allele to generate the extrachromosomal array lsEx296[ife-4::NLS::GFP rol-6(su1006)]. A second ife-4::GFP fusion was created as described above except that pPD95.67[Delta]NLS (the NLS was deleted by KpnI digestion and religation) was used in PCR. For microinjections, the second fusion product was mixed with linearized C. elegans genomic DNA to generate the complex extrachromosomal array lsEx385[ife-4::GFP rol-6(su1006)]. --precise ends. | ||
| Pattern | Both transgenes were expressed in pharyngeal neurons and muscle, ventral nerve cord (VNC), vulval neurons and muscle, tail neurons, and spermatheca sheath. Expression occurred from late embryo to adult, especially in the pharynx. Other tissues, such as vulva and tail, had increased fluorescence during the late L3 and L4 stages. ife-4::GFP expression was not detected in germ line or somatic gonadal tissues other than spermatheca. | |||
| Reference | WBPaper00024654 | |||
| Transgene | WBTransgene00000896 | |||
| WBTransgene00000895 |
