WormBase Tree Display for Microarray_experiment: [cgc5303]:SL2_enriched
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[cgc5303]:SL2_enriched | Microarray | SMD2 |
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Species | Caenorhabditis elegans | |
Sample (2) | ||
Reference | WBPaper00005303 | |
Remark | Cy3-dUTP was used to label SL2-enriched cDNA and Cy5-dUTP was used to label cDNA from poly(A)+ RNA made from a mixed stage population of wild-type worms. The SL2-enriched probe and the probe from the starting poly(A)+ mRNA were simultaneously hybridized to DNA microarrays. To ensure reproducibility, this procedure was repeated five times. Ratios of Cy3/Cy5 (SL2/poly(A)+) signals were calculated for each gene and converted to log2(ratio). The average log2(ratio) from the five repeats was then calculated. |