WormBase Tree Display for Construct: WBCnstr00018008
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| WBCnstr00018008 | Public_name | pGLC44 | |
|---|---|---|---|
| Summary | [hda-1::gfp] | ||
| Driven_by_gene | WBGene00001834 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | This hda-1::gfp plasmid was made by subcloning an NsiI/HindIII-digested 1,024 bp DNA fragment from C. elegans into the Fire lab vector pPD95.69 (polymerase chain reaction primers GL363/GL360). Because the C. elegans fragment contains all but approximately 250 bp of the DNA region between hda-1 and its upstream gene, ril-1, it is formally possible that gfp expression in the transgenic animals is regulated by both the hda-1 and ril-1 enhancers. However, this is unlikely because ril-1 has no known function in vulva and uterine cells (HANSEN et al. 2005; LEMIRE et al. 2009). | ||
| Used_for | Transgene_construct | WBTransgene00018643 | |
| Reference | WBPaper00043890 |
