small RACE products -pool E

Mixed-stage 3'RACE 454 sequencing & alignment (Piano project, Piano subgroup)

General Description

The 3' untranslated region (3'UTR) constitutes a major site of post-transcriptional regulation of gene expression. Sequence elements in the 3'UTR interact with trans-acting regulators such as microRNAs that affect translation and stability. The overall aim is to use a 3'RACE cloning-sequencing stragety to identify the 3'UTRs of C. elegans transcripts and explore their heterogeneity in different developmental stages and tissues.


  1. Growth and isolation: Single colony DNA extraction
  2. Sample preparation: Single colony DNA extraction, 454 sequencing
  3. Data Analysis: 454 Data Processing
  1. External Links: GSM469976

Related modENCODE submissions:

Release Date: 2009-11-16