TSS (Chen) Reverse

This is a set of Transcription Start Sites (TSS) in the Reverse sense identified in short read data from the paper:
The landscape of RNA polymerase II transcription initiation in C. elegans reveals promoter and enhancer architectures.Chen RA, Down TA, Stempor P, Chen QB, Egelhofer TA, Hillier LW, Jeffers TE, Ahringer J. Genome Res. 2013 Jun 20.
PubMed 23550086

The RNASeq data was obtained from the NCBI Sequence Read Archive (SRA) ( http://www.ncbi.nlm.nih.gov/sra) under accession number SRA062711. This was aligned to the current version of the assembly and the number of reads aligned to the reverse sense of the genome were counted at the genomic position of the 5' end of the aligned read. The count at each genomic position was then normalised by dividing by the number of millions of aligned reads, giving a value in units of Reads Per Million (RPM).