[Pmex-5::ATX-2a::GFP::
tbb-2 3' UTR +
unc-119(+)] II was generated as follows: cDNA of the a isoform of
atx-2 (
atx-2a) was amplified from pT7 (generously provided by Rafal Ciosk, Friedrich Miescher Institute for Biomedical Research) and then inserted into pCR8/GW/TOPO (Invitrogen, Carlsbad, CA). The primers used to amplify the
atx-2a were sense primer, 5'-ATGTCAACACCAACGGGTCTTCCTGCG-3', and antisense primer, 5'-CGGGGGGCTCTGAGAGTGCTG-3'.