Figure 3. Expression of
ceh-51. (A,B)
ceh-51 transcripts occur in (A) the MS daughter cells (MS2) and (B) in the MS granddaughters (MS4), as detected by in situ hybridization. The E daughters are outlined. Ninety-one percent (n=101) of embryos at this stage showed expression in MS2 or MS4 (nine embryos did not stain). (C) Ectopic expression of
ceh-51 following heat shock of hs-
tbx-35 embryos. (D) Eighty-six percent (n=44) of
pop-1(RNAi) embryos showed
ceh-51 mRNA in both the MS and E daughters. Two embryos showed normal expression and four embryos did not stain. (E) Embryos transgenic for a
ceh-51::GFP transcriptional reporter with 788 bp of upstream sequence show expression at MS4 that persists in later MS descendants. (F) A translational
ceh-51::GFP::CEH-51 fusion shows strong nuclear accumulation at MS8. (G)
ceh-51::GFP is undetectable in
med-1(
ok804);
med-2(
cx9744) embryos (n=84). (H) Sixty-six percent (n=41) of
pop-1(RNAi) embryos showed
ceh-51::GFP in both the MS and E lineages (the remainder were similar to wild type). (I,J)
mex-1(RNAi) (I) and
pie-1(RNAi) (J) embryos displayed ectopic
ceh-51::GFP in AB and C descendants. (K) In
tbx-35(
tm1789) embryos, the onset of
ceh-51::GFP expression was undetectable (52%, n=89) or delayed until past the MS8 stage (48%) and at lower levels. The exposure in this image was 10-fold longer than that shown in E. (L)
ceh-51::GFP was not detected in
tbx-35(
tm1789);
pop-1(RNAi) embryos (n=49).