Figure 4.
del-1::GFP and
acr-5::GFP are expressed ectopically in A-type motor neurons of
unc-4 and
unc-37 mutants. GFP expression (green) was visualized in mid- to late L2 larval animals. Autofluorescent gut granules are yellow. (A-F) The
del-1::gfp reporter gene is regulated by 1.8 kb of
del-1 upstream sequence. In the ventral nerve cord of wild-type L2 animals,
del-1::GFP is expressed in the VB motor neurons but is prematurely expressed in the VAs during this period in
unc-37(
e262) and in
unc-4 mutants. (G-L) The
acr-5::gfp reporter gene is regulated by 4.2 kb of
acr-5::gfp upstream sequence.
acr-5::GFP is expressed in B-type motor neurons (DB, VB) in ventral nerve cords of wild-type animals. In
unc-4 and
unc-37 mutants,
acr-5::GFP is ectopically expressed in A-type motor neurons (DA, VA). Genotypes for each row are indicated at left.
unc-37(
e262) corresponds to UNC-37(H539Y);
unc-4(
e2321) corresponds to UNC-4(R197K). (C,I) Enlargements of areas indicated by insets in B and H, respectively. Unlabeled motor neurons in E, F, K, and L are identified at top. Bars, 20 μm. Bar in B applies to B and H; bar in C applies to C-L.