Fig 3. UNC-44 acts upstream of UNC-33. Representative images of immunostaining results for FLAG::UNC-33(S)(A) and UNC-44(B) in control and mutant animals. UNC-33(S) localization was determined by a transgene expressing FLAG tagged UNC-33(S) in all neurons (Prgef-1::FALG::
unc-33(S)). (C) Overexpression of UNC-33(S) suppresses
unc-44(lf) phenotypes. Each experiment was performed with N>200 animals at least three times. For transgenic animals the results shown here are generated from at least three independent lines. Data are shown as mean +- SD. Student's t-test, ** p<0.01. Scale bar: 10 um.