Figure 4. ANI-1 is required for the formation of cortical patches containing myosin II and the septins. (A) Wild-type embryos and embryos depleted of ANI-1, both UNC-59 and UNC-61, or NMY-2 were fixed prior to pronuclear migration (when the anterior cortex normally ruffles) and stained for ANI-1, NMY-2 and UNC-59. Ruffles are not preserved by the fixation. In wild-type embryos, all three proteins concentrate in an inhomogeneous network of cortical patches. In
ani-1(RNAi) embryos, UNC-59 and NMY-2 are diffuse on the cortex. Scale bar: 10 um. (B) Schematic showing region of embryo magnified in C. (C) Magnified view of the cortex from control and
ani-1(RNAi) embryos in A. Scale bar: 5 um.